Regulatory

Part:BBa_K3669005:Design

Designed by: Paul Osuna-Kleist   Group: iGEM20_UC_Davis   (2020-10-27)


Pcox4 Constitutive Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 721
    Illegal NotI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 972
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 721
    Illegal NgoMIV site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adds promoter diversity in our construct since we already are using gpdA3B. Had some BsaI and SapI sites that needed to be changed (will be annotated in sequence).


Source

Directly from promoters tested from Aspergillus Niger. https://link.springer.com/article/10.1007%2Fs00253-012-4207-9

References